BIOTECHNOLOGY & GENETIC ANALYSIS PRACTICAL ASSESSMENT Essay

BIOTECHNOLOGY & GENETIC ANALYSIS PRACTICAL ASSESSMENT - Essay caseAs most of these kind of catabolic genes found on plasmid observed b proceedingerial community obtains from origin of river having very few plasmids. While in case of second sample which was obtained from nearby settle of UWIC having large number of plasmid bearing bacteria. This is mainly attributed to accumulation of pollutant along the path of river and hence bacterial population need to evolved catabolic capability to survive and hence more plasmid bearing bacterial population were found in second sample. Similarly most of the plasmid was found to be in size range more than 35 KB clearly indicates most of them are Conjugative plasmids as this theme of plasmid has more number of genes compared to non-conjugative plasmid to carryout conjugation process and hence larger the size.Re-suspension solution constitutes of glucose, EDTA and Tris to each one have its own role. Glucose provides osmotic stress and EDTA as chelating agents which binds to heavy metals and helps in disintegration of cell wall. Tris act as buffering agent and maintains pH of over all reactions to avoid any pH dependent military position reaction. In this stage cells become highly fragile and some are break open.This solution is variety show of SDS and NaOH.